Vesper Show more Add to Mendeley Share Cite Get rights and content Under an Elsevier user license open archive Highlights An accurate, specific, high-throughput method was developed for measuring creatinine in urine.One minute chromatographic run achieved separation of creatinine from creatine.
Statistical Methods George W Snedecor William G Cochran Serial Dilution StepsFully automated sample preparation included vial decapping, scanning, and serial dilution steps.
Statistical Methods George W Snedecor William G Cochran Archive Highlights AnThe method is suitable for routine measurements of large epidemiological studies. A high-throughput, accurate liquid chromatography tandem mass spectrometry method for quantitation of human urinary creatinine has been developed and validated. Sample preparation was fully automated including cryovial decapping, sample ID scanning and two serial dilution steps. Quantitation was performed using a stable isotope-labeled internal standard. Multiplexed chromatographic separation of creatinine was achieved within a one-minute analysis and followed by tandem mass spectrometry in positive electrospray ionization mode. The precursor and product ions of creatinine and D 3 -creatinine were monitored in selected reaction monitoring mode. Method validation results showed reproducibility with within-run precision of 3.59, 3.49 and 2.84 and between-run precision of 4.01, 3.28 and 3.57 for low, medium and high quality control materials prepared from pooled donor urine, respectively. ![]() A simple, accurate, high-throughput method was successfully developed for measuring creatinine in human urine samples. Previous article in issue Next article in issue Abbreviations CI Confirmation ion CV coefficient of variation HESI heated electrospray ionization IRB institutional review board LC-MSMS liquid chromatography tandem mass spectrometry LOD limit of detection LLOQ lower limit of quantitation ME matrix effects NIST National Institute of Standards and Technology QI quantitation ion QC quality control SD standard deviation SRM standard reference material UHPLC ultra-high performance liquid chromatography Keywords Mass spectrometry Urine creatinine Automation High throughput Recommended articles Citing articles (0) View Abstract Published by Elsevier B.V. The Association for Mass Spectrometry: Applications to the Clinical Lab (MSACL). Citing articles Article Metrics View article metrics About ScienceDirect Remote access Shopping cart Advertise Contact and support Terms and conditions Privacy policy We use cookies to help provide and enhance our service and tailor content and ads. Copyright 2021 Elsevier B.V. ScienceDirect is a registered trademark of Elsevier B.V.
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